rabbit anti serpine1  (Proteintech)

Journal: Frontiers in Oncology

Article Title: Loss of TARBP2 Drives the Progression of Hepatocellular Carcinoma via miR-145-SERPINE1 Axis

doi: 10.3389/fonc.2021.620912

Figure Lengend Snippet: Differential genes after overexpression and knockdown of TARBP2 in HepG2 cells. (A) Heatmaps represent the expression level of the whole genome (left) and showing the intersect of differentially expressed genes at 70% fold-change from two groups (right) respectively. (oe-Ctrl vs oe- TARBP2 , and sh-Ctrl vs sh- TARBP2 ). (B) A Venn Diagram of differentially expressed genes at the cut-off of 70% fold-change. Group-I, up-regulated genes from oe-Ctrl to oe- TARBP2 ; Group-II, down-regulated genes from oe-Ctrl to oe- TARBP2 ; Group-III, up-regulated genes from sh-Ctrl to sh- TARBP2 ; Group-IV, down-regulated genes from sh-Ctrl to sh- TARBP2 . (C) The KEGG pathways and GO biological processes enriched in the differentially expressed genes at 70% fold-change. (D) The mRNA expression levels of candidate genes from enriched pathway. (E) Relative expression levels of SERPINE1 in HCC tissues. (F) Correlation between the relative expression level of TARBP2 and SERPINE1 in HCC tissues. *p-value < 0.05.

Article Snippet: The membranes were blocked in 5% non-fat milk followed by overnight incubation with primary antibodies including rabbit anti-TRBP (15753-1-AP; ProteinTech, Wuhan, China), rabbit anti-PAI-1 (SERPINE1) (13801-1-AP; ProteinTech, Wuhan, China) and rabbit anti-alpha TUBULIN (11224-1-AP; ProteinTech, Wuhan, China).

Techniques: Over Expression, Knockdown, Expressing

Journal: Frontiers in Oncology

Article Title: Loss of TARBP2 Drives the Progression of Hepatocellular Carcinoma via miR-145-SERPINE1 Axis

doi: 10.3389/fonc.2021.620912

Figure Lengend Snippet: SERPINE1 is a downstream player of TARBP2 . (A, B) The mRNA and protein expression of TARBP2 and SERPINE1 in the stable sh- TARBP2 HepG2 cells after treatment with scramble control and sh- SERPINE1 . (C) Cell migration; (D) Invasion; and (E) Proliferation assays of sh- TARBP2 HepG2 cells treated with scramble control and sh- SERPINE 1. *p-value < 0.05; **p-value < 0.01.

Article Snippet: The membranes were blocked in 5% non-fat milk followed by overnight incubation with primary antibodies including rabbit anti-TRBP (15753-1-AP; ProteinTech, Wuhan, China), rabbit anti-PAI-1 (SERPINE1) (13801-1-AP; ProteinTech, Wuhan, China) and rabbit anti-alpha TUBULIN (11224-1-AP; ProteinTech, Wuhan, China).

Techniques: Expressing, Control, Migration

Journal: Frontiers in Oncology

Article Title: Loss of TARBP2 Drives the Progression of Hepatocellular Carcinoma via miR-145-SERPINE1 Axis

doi: 10.3389/fonc.2021.620912

Figure Lengend Snippet: SERPINE1 mRNA is a target of miRNA-145 linked to TARBP2 . (A) Flow chart of selection and analysis of potential miRNAs. (B) Levels of 4 potential miRNAs in the oe/sh- TARBP2 HepG2 cells. (C) Comparison of miR-145 expression between HCC tumors and tumor adjacent tissues (N = 20) (paired t-test p-value = 0.038). (D, E) miR-145 levels in HCC tumors are positively associated with TARBP2 (Pearson r = 0.47, p-value = 0.037) and negatively associated with SERPINE1 (Pearson r = -0.45, p-value = 0.049) (N=20). (F) The binding sites of miR-145 in the 3’-UTR of SERPINE1 mRNA. (G) The effects of miR-145 on reporters, pmirGLO- SERPINE1 -wild-type and pmirGLO- SERPINE1 -mutant, were measured by luciferase reporter assays in HepG2 cells. *p-value < 0.05; **p-value < 0.01; NS, no significance; NC, negative control.

Article Snippet: The membranes were blocked in 5% non-fat milk followed by overnight incubation with primary antibodies including rabbit anti-TRBP (15753-1-AP; ProteinTech, Wuhan, China), rabbit anti-PAI-1 (SERPINE1) (13801-1-AP; ProteinTech, Wuhan, China) and rabbit anti-alpha TUBULIN (11224-1-AP; ProteinTech, Wuhan, China).

Techniques: Selection, Comparison, Expressing, Binding Assay, Mutagenesis, Luciferase, Negative Control

Journal: Frontiers in Oncology

Article Title: Loss of TARBP2 Drives the Progression of Hepatocellular Carcinoma via miR-145-SERPINE1 Axis

doi: 10.3389/fonc.2021.620912

Figure Lengend Snippet: miR-145 mediates SERPINE1 to affect the role of TARBP2 in HCC progression. (A) Levels of miR-145 in sh- TARBP2 HepG2 cells after treatment with Mimic NC and Mimic miR-145. (B) Protein levels of SERPINE1 and TARBP2 in the stable sh- TARBP2 HepG2 cells after treatment with Mimic NC and Mimic miR-145. (C) Cell migration; (D) Invasion; and (E) Proliferation of sh- TARBP2 HepG2 cells treated with Mimic NC and Mimic miR-145. *p-value < 0.05; **p-value < 0.01; NC, negative control.

Article Snippet: The membranes were blocked in 5% non-fat milk followed by overnight incubation with primary antibodies including rabbit anti-TRBP (15753-1-AP; ProteinTech, Wuhan, China), rabbit anti-PAI-1 (SERPINE1) (13801-1-AP; ProteinTech, Wuhan, China) and rabbit anti-alpha TUBULIN (11224-1-AP; ProteinTech, Wuhan, China).

Techniques: Migration, Negative Control

Journal: Frontiers in Oncology

Article Title: Loss of TARBP2 Drives the Progression of Hepatocellular Carcinoma via miR-145-SERPINE1 Axis

doi: 10.3389/fonc.2021.620912

Figure Lengend Snippet: A working model for the TARBP2-miR-145-SERPINE1 axis in HCC progression. The loss of TARBP2 induces the upregulation of SERPINE1 through reducing the processing of miR-145. This linear TARBP2-miR-145-SERPINE1 axis is involved in proliferation, migration, and invasion in HCC cells.

Article Snippet: The membranes were blocked in 5% non-fat milk followed by overnight incubation with primary antibodies including rabbit anti-TRBP (15753-1-AP; ProteinTech, Wuhan, China), rabbit anti-PAI-1 (SERPINE1) (13801-1-AP; ProteinTech, Wuhan, China) and rabbit anti-alpha TUBULIN (11224-1-AP; ProteinTech, Wuhan, China).

Techniques: Migration